(1) Immunogenicity and Pre-Clinical Efficacy of an OMV-Based SARS-CoV-2 VaccineVaccines (Basel)September 29, 2023Alberto Grandi, Michele Tomasi, Irfan Ullah, Cinzia Bertelli, Teresa Vanzo, Silvia Accordini, Assunta Gagliardi, Ilaria Zanella, Mattia Benedet, Riccardo Corbellari, et al.gentleMACS dissociator (Miltenyi Biotec, #130-096-427, Bergisch Gladbach, Germany ) with program RNA_02 in M tubes (#130-096-335) in 1 mL of Trizol (Invitrogen, #15596018, Waltham, MA, USA). The homogenates were centrifuged at 2000×

(2) Attenuated Mycobacterium tuberculosis vaccine protection in a low-dose murine challenge modeliScienceJune 16, 2023Samuel J. Vidal, Daniel Sellers, Jingyou Yu, Shoko Wakabayashi, Jaimie Sixsmith, Malika Aid, Julia Barrett, Sage F. Stevens, Xiaowen Liu, Wenjun Li, et al.lung lobes were dissected separately. Tissues were placed into gentleMACS M Tubes (Miltenyi Biotec 130-096-335) containing 5 mL of PBS and mechanically dissociated using a gentleMACS Dissociator (Miltenyi Biotec).

(3) Nirmatrelvir treatment of SARS‐CoV‐2‐infected mice blunts antiviral adaptive immune responsesEMBO Molecular MedicineMay 8, 2023Valeria Fumagalli, Pietro Di Lucia, Micol Ravà, Davide Marotta, Elisa Bono, Stefano Grassi, Lorena Donnici, Rolando Cannalire, Irina Stefanelli, Anastasia Ferraro, et al.turbinates using gentleMACS dissociator (Miltenyi BioTec, #130‐096‐427) with program RNA_02 in M tubes (#130‐096‐335) in 1 ml of Trizol (Invitrogen, #15596018). The homogenates were centrifuged at 2,000 g for 1 min at

(4) Breaking the crosstalk of the Cellular Tumorigenic Network by low-dose combination therapy in lung cancer patient-derived xenograftsCommunications BiologyDecember 1, 2022Dennis GÃ¼rgen, Theresia Conrad, Michael Becker, Susanne Sebens, Christoph RÃ¶cken, Jens Hoffmann, Stefan Langhammerwas disrupted in 1.5 ml TRIzolTM using a gentleMACSTM dissociator and M tubes (Cat-No. 130-093-235 & 130-096-335, Miltenyi Biotec, Germany). RNA integrity was evaluated with Agilent Bioanalyzer 2100 and RNA 6000 Nano

(5) DNA barcoding reveals ongoing immunoediting of clonal cancer populations during metastatic progression and immunotherapy responseNat CommunNovember 7, 2022Louise A. Baldwin, Nenad Bartonicek, Jessica Yang, Sunny Z. Wu, Niantao Deng, Daniel L. Roden, Chia-Ling Chan, Ghamdan Al-Eryani, Damien J. Zanker, Belinda S. Parker, et al.samples were lysed in 5 ml QIAGEN buffer P1 (with RNaseA) and 0.5% SDS within a Miltenyi M-Tube (# 130-096-335). Samples were processed on the gentleMACS or gentleMACS Octo using the RNA_02 programme. DNA was then

(6) Optimized intramuscular immunization with VSV-vectored spike protein triggers a superior immune response to SARS-CoV-2NPJ vaccinesJuly 25, 2022Taddeo A, Veiga IB, Devisme C, Boss R, Plattet P, Weigang S, Kochs G, Thiel V, Benarafa C, Zimmer G.and the distal half of the right brain hemisphere were homogenized in Tube M (Miltenyi Biotech, ref. 130-096-335) containing 1 ml of DMEM using a gentleMACS™ Tissue Dissociator (Miltenyi Biotech). Viral titers were

(7) Enhanced fitness of SARS-CoV-2 variant of concern Alpha but not BetaNatureFebruary 1, 2022Ulrich L, Halwe NJ, Taddeo A, Ebert N, Schön J, Devisme C, Trüeb BS, Hoffmann B, Wider M, Fan X, et al.β-mercaptoethanol using a Bullet Blender Tissue Homogenizer (Next-Advance) or in Tube M (Miltenyi Biotech, 130-096-335) containing 1 ml of DMEM using a gentleMACS Tissue Dissociator (Miltenyi Biotech). Nucleic acid was

(8) Cardiac cell type–specific gene regulatory programs and disease risk associationScience AdvancesMay 14, 2021James D. Hocker et al.samples were homogenized using a gentleMACS dissociator (Miltenyi) with gentleMACS M tubes (Miltenyi, 130-096-335) and the Protein_01_01 protocol. Suspension was filtered through a 30 M CellTrics filter (Sysmex, 04-0042-2316).

(9) Vav2 catalysis-dependent pathways contribute to skeletal muscle growth and metabolic homeostasisNature communicationsNovember 16, 2020Rodríguez-Fdez S, Lorenzo-Martín LF, Fernández-Pisonero I, Porteiro B, Veyrat-Durebex C, Beiroa D, Al-Massadi O, Abad A, Diéguez C, Coppari R, et al.help of a Dispomix Drive homogenizer (Cat. #900020.00, Medic Tools AG) in gentleMACS M tubes (Cat. #130-096-335, Miltenyi Biotec) in ice-cold lysis buffer [50 mM Tris-HCl (pH 7.5), 1 mM ethylene glycol tetraacetic

(10) VAV2 signaling promotes regenerative proliferation in both cutaneous and head and neck squamous cell carcinomaNature communicationsSeptember 22, 2020Lorenzo-Martín LF, Fernández-Parejo N, Menacho-Márquez M, Rodríguez-Fdez S, Robles-Valero J, Zumalave S, Fabbiano S, Pascual G, García-Pedrero JM, Abad A, et al.gentleMACS dissociator (Miltenyi Biotec, Catalog No. 130-093-235) and gentleMACS M tubes (Myltenyi Biotec, Catalog No. 130-096-335). Extracts were precleared by centrifugation at 13,200 rpm for 10 min at 4 °C. Protein concentration

(11) Vav2 pharmaco-mimetic mice reveal the therapeutic value and caveats of the catalytic inactivation of a Rho exchange factorOncogeneJuly 9, 2020L. Francisco Lorenzo-Martín, Sonia Rodríguez-Fdez, Salvatore Fabbiano, Antonio Abad, María C. García-Macías, Mercedes Dosil, Myriam Cuadrado, Javier Robles-Valero, Xosé R. BustelogentleMACS dissociator (Miltenyi Biotec, Catalog No. 130-093-235) and gentleMACS M tubes (Myltenyi Biotec, Catalog No. 130-096-335). Extracts were precleared by centrifugation at 13 200 rpm for 30 min at 4 °C and supernatants quantified

(12) A whole-tissue RNA-seq toolkit for organism-wide studies of gene expression with PME-seqNature ProtocolsApril 1, 2020Surya Pandey, Michihiro Takahama, Adam Gruenbaum, Makda Zewde, Katerina Cheronis, Nicolas ChevrierPCRAS200) 25 ml Combitip (VWR, cat. no. 0030089472) gentleMACS M tubes (Miltenyi Biotec, cat. no. 130-096-335) 0.5 ml 96-well deep well polypropylene plate (USA Scientific, cat. no. 1896-5000) 2.0 ml 96-well

(13) Inhibition of NOX2 signaling limits pain-related behavior and improves motor function in male mice after spinal cord injury: Participation of IL-10/miR-155 pathwaysBrain, Behavior, and ImmunityAugust 1, 2019Boris Sabirzhanov, Yun Li, Marino Coll-Miro, Jessica J. Matyas, Junyun He, Alok Kumar, Nicole Ward, Jingwen Yu, Alan I. Faden, Junfang Wususpension were incubated with CD11b microbeads (130-093-634, Miltenyi Biotec) and loaded onto a MS column (130-096-335, Miltenyi Biotec) placed in the magnetic field of a MACS separator. The negative fraction (flow through)

(14) Prophylactic TLR9 stimulation reduces brain metastasis through microglia activationPLOS BiologyMarch 28, 2019Amit Benbenishty et al.the manufacturer’s instructions. Whole organs were homogenized in denaturation solution A in M tubes (130-096-335, Milteny Biotec) by gentleMACS dissociator (Milteny Biotec). Reverse transcription was performed with

(15) Androgen Receptor Pathway-Independent Prostate Cancer Is Sustained through FGF SignalingCancer cellOctober 9, 2017Bluemn EG, Coleman IM, Lucas JM, Coleman RT, Hernandez-Lopez S, Tharakan R, Bianchi-Frias D, Dumpit RF, Kaipainen A, Corella AN, et al.Stranded mRNA Library Preparation Kit,Illumina,Cat# RS-122-2101 gentleMACS M Tubes,Miltenyi Biotec,Cat# 130-096-335 RNA STAT-60,Tel-Test,Cat# Cs-502 Critical Commercial Assays,, "CultreCoat Low BME Cell Invasion Assay, 96 well

(16) Organism-Level Analysis of Vaccination Reveals Networks of Protection across TissuesCellOctober 5, 2017Kadoki M, Patil A, Thaiss CC, Brooks DJ, Pandey S, Deep D, Alvarez D, von Andrian UH, Wagers AJ, Nakai K, et al.(Qiagen). For liver and small intestine samples, tissues were lysed using M tubes (Miltenyi biotec 130-096-335) and running 1–4 cycles of the RNA_02.01 program on the gentleMACS octo dissociator (Miltenyi biotec).

(17) New insights into experimental visceral leishmaniasis: Real-time in vivo imaging of Leishmania donovani virulencePLOS Neglected Tropical DiseasesSeptember 25, 2017Guilherme D. Melo, Sophie Goyard, Hervé Lecoeur, Eline Rouault, Pascale Pescher, Laurence Fiette, Alexandre Boissonnas, Paola Minoprio, Thierry Langcollection Infected hamsters and mice were euthanized, the spleens were collected in gentleMACS M Tubes (130-096-335, Miltenyi Biotec, Bergisch Gladbach, Germany) containing 5 mL of supplemented M199 medium and homogenized

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M Tubes

gentleMACS M Tubes (4x 25) from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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gentleMACS M Tubes (4x 25) from Miltenyi Biotec

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